Abolfazl Rad; Seyyed Mehdi Beheshti nasr; Hasan Ramshini
Volume 21, Issue 5 , September and October 2014, , Pages 711-718
Abstract
Background and purpose: Minocycline has got the anti-inflammatory and neuroprotective effects. Considering the interaction between cell death and seizure, and on the other hand, Kindling which increases expression NMDA receptors in brain, the aim of this study is to investigate the effect of minocycline ...
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Background and purpose: Minocycline has got the anti-inflammatory and neuroprotective effects. Considering the interaction between cell death and seizure, and on the other hand, Kindling which increases expression NMDA receptors in brain, the aim of this study is to investigate the effect of minocycline on gene expression of NMDA receptor in hippocampus and piriform brain areas on amygdale kindling acquisition in rats.
Materials and Methods: In this experimental study, three animal groups of 24 Wistar rats received kindling stimulations (twice daily within 6 hours intervals) after being stereotaxic operated and taking one week recovery period. In first Group (n=8) animals did not received daily kindling stimulations. Animals of the second and the third Groups (n=8) respectively had been injected by saline (1ml/kg) and minocycline (25 mg/kg), 60 minutes before receiving kindling stimulations. Two hours after last stimulation animal’s brains were removed and the changes of NR2A gene subunit of NMDA receptor in the hippocampus and piriform cortex were measured and compared relative to the control group. Datawere analyzed using ANOVA and Tukey post hoc tests at significant level of P
Shahin Ramazi; Majid Motovalli Bashi; Abolfazl Rad; Hamidreza Khezraee; Morteza Hashemzadeh Chaleshtari
Volume 21, Issue 5 , September and October 2014, , Pages 842-848
Abstract
Background: Allergic rhinitis is a chronic inflammatory disease of the upper airways, this inflammatory response in the nasal mucosa includes an immediate IgE-mediated response. IL-18, a member of the IL-1 family, is known for influencing the balance of IgE level. In present study this problem is investigated ...
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Background: Allergic rhinitis is a chronic inflammatory disease of the upper airways, this inflammatory response in the nasal mucosa includes an immediate IgE-mediated response. IL-18, a member of the IL-1 family, is known for influencing the balance of IgE level. In present study this problem is investigated that whether immunoglobulin (Ig) E levels in serum are associated with -607A/C SNPs of IL18 promoter.
Materials and Methods: Genotyping for -607A/C SNPs of IL18 promoter was performed using 133 patients with AR and 62 healthy control volunteers from Chaharmahal va Bakhtiari province then serum levels of total IgE were determined by ELISA method. Statistical analysis were carried out using T test and ANOVA test by SPSS version 19.
Results: level of total IgE in Serum were significantly greater in allergic rhinitis patients than controls (P=0.017). In addition, the total serum IgE level of the individuals with heterozygous genotype of IL18 (-607AC) were significantly higher regarding other investigated polymorphisms (P=0.045).
Conclusion: According to the results of this study, it seems that the IL18 gene polymorphism -607A/C is associated with IgE levels and susceptibility of allergic rhinitis.